Long non-coding RNA BCAR4 aggravated proliferation and migration in esophageal squamous cell carcinoma by negatively regulating p53/p21 signaling pathway

Shuo Yan; Jichong Xu; Bingyan Liu; Lin Ma; Hao Feng; Huaqiao Tan; Chun Fang

doi:10.1080/21655979.2021.1887645

Long non-coding RNA BCAR4 aggravated proliferation and migration in esophageal squamous cell carcinoma by negatively regulating p53/p21 signaling pathway

Bioengineered. 2021 Dec;12(1):682-696. doi: 10.1080/21655979.2021.1887645.

Authors

Shuo Yan¹, Jichong Xu¹, Bingyan Liu², Lin Ma¹, Hao Feng¹, Huaqiao Tan¹, Chun Fang¹

Affiliations

¹ Department of Interventional Radiology, Tongji Hospital of Tongji University , Shanghai, China.
² Department of Interventional Radiology, Tongren Hospital, Shanghai Jiaotong University School of Medicine , Shanghai, China.

Abstract

Long non-coding RNA breast cancer antiestrogen resistance 4 (lncRNA BCAR4) is an independent factor on the survival prognosis of patients with multiple cancers. However, the role of lncRNA BCAR4 in esophageal squamous cell cancer (ESCC) remains unknown. Here, we unraveled that lncRNA BCAR4 was upregulated in ESCC and predicted poor prognosis. Functionally, lncRNA BCAR4 knockdown induced cell apoptosis and G1/S arrest, while inhibited cell proliferation and migration in vitro; conversely, overexpressing lncRNA BCAR4 promoted proliferation and metastasis. Mechanistically, lncRNA BCAR4 sponged miR-139-3p to upregulate ELAVL1, thereby inhibiting p53/p21 pathway in ESCC cells. In conclusion, lncRNA BCAR4 promotes ESCC tumorigenesis via regulating p53/p21 signaling pathway and develops a brand-new biomarker and medicine target for ESCC.

Keywords: ELAVL1; ESCC; LncRNA BCAR4; miR-139-3p; p53/p21 signaling pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / genetics
Cell Line, Tumor
Cell Movement / genetics*
Cell Proliferation / genetics*
Cyclin-Dependent Kinase Inhibitor p21 / genetics
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
Esophageal Neoplasms* / genetics
Esophageal Neoplasms* / metabolism
Esophageal Neoplasms* / mortality
Esophageal Neoplasms* / pathology
Esophageal Squamous Cell Carcinoma* / genetics
Esophageal Squamous Cell Carcinoma* / metabolism
Esophageal Squamous Cell Carcinoma* / mortality
Esophageal Squamous Cell Carcinoma* / pathology
Female
Gene Expression Regulation, Neoplastic / genetics
Humans
Mice
Mice, Inbred BALB C
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
Signal Transduction / genetics
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism

Substances

BCAR4 non-coding RNA, human
CDKN1A protein, human
Cyclin-Dependent Kinase Inhibitor p21
RNA, Long Noncoding
TP53 protein, human
Tumor Suppressor Protein p53

Grants and funding

National Natural Science Foundation of China (81702872 to S.Y. and 81702942 to B.L.), Shanghai Natural Science Fund Project (Grant no. 19ZR1449000), General Program of Shanghai Municipal Commission of Health and Family Planning (No. 20174Y0095) provide finance foundation to authors.