Experimental Model of Human Malignant Mesothelioma in Athymic Mice

Int J Mol Sci. 2018 Jun 26;19(7):1881. doi: 10.3390/ijms19071881.

Abstract

Malignant pleural mesothelioma (MPM) is a thoracic aggressive cancer caused by asbestos exposure, which is difficult to diagnose and treat. Here, we characterized an in vivo orthotopic xenograft model consisting of human mesothelioma cells (designed as H2052/484) derived from a pleural NCI-H2052 tumor injected in partially immunodeficient athymic mice. We assessed tumor formation and tumor-dependent patterns of inflammation. H2052/484 cells conserved their mesothelioma phenotype and most characteristics from the parental NCI-H2052 cells. After intra-thoracic injection of H2052/484 cells, thoracic tumors developed in nearly all mice (86%) within 14 days, faster than from parental NCI-H2052 cells. When the mice were euthanized, the pleural lavage fluid was examined for immune cell profiles. The pleural immune cell population increased with tumor development. Interestingly, the proportion of myeloid-derived suppressor cell and macrophage (especially CD206⁺ M2 macrophages) populations increased in the pleural fluid of mice with large mesothelioma development, as previously observed in immunocompetent mice. This reliable orthotopic model recapitulates human mesothelioma and may be used for the study of new treatment strategies.

Keywords: athymic mouse; cancer; immune cells; mesothelioma; orthotopic xenotransplantation; pleura.

MeSH terms

  • Animals
  • Body Fluids / metabolism
  • Carcinogenesis / pathology
  • Cell Count
  • Cell Line, Tumor
  • Cell Survival
  • Epithelial-Mesenchymal Transition / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms / genetics
  • Lung Neoplasms / immunology
  • Lung Neoplasms / pathology*
  • Macrophages / metabolism
  • Mesothelioma / genetics
  • Mesothelioma / immunology
  • Mesothelioma / pathology*
  • Mesothelioma, Malignant
  • Mice, Nude
  • Xenograft Model Antitumor Assays*