Ion Binding to Transport Proteins using Isothermal Titration Calorimetry

Shian Liu; Steve W Lockless

doi:10.1007/978-1-4939-7362-0_22

Ion Binding to Transport Proteins using Isothermal Titration Calorimetry

Methods Mol Biol. 2018:1684:289-303. doi: 10.1007/978-1-4939-7362-0_22.

Authors

Shian Liu^{1

2}, Steve W Lockless³

Affiliations

¹ Department of Biology, Texas A&M University, 3474 TAMU, College Station, TX, 77843-3474, USA.
² Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA, 20148, USA.
³ Department of Biology, Texas A&M University, 3474 TAMU, College Station, TX, 77843-3474, USA. lockless@bio.tamu.edu.

PMID: 29058200
DOI: 10.1007/978-1-4939-7362-0_22

Abstract

Isothermal titration calorimetry (ITC) is an emerging, label-free technology used to measure ligand binding to membrane proteins. This technology utilizes a titration calorimeter to measure the heat exchange upon ligands binding to proteins, the magnitude of which is based on the overall enthalpy of the reaction. In this protocol, the steps we and others use to measure ion binding to ion transport proteins are described.

Keywords: Channel; Equilibrium; Ion binding; Isothermal titration calorimetry; Transporter.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calorimetry
Carrier Proteins / metabolism*
Hot Temperature
Ions / metabolism*
Protein Binding
Thermodynamics

Substances

Carrier Proteins
Ions