Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9

Stem Cell Res. 2017 Jul:22:29-32. doi: 10.1016/j.scr.2017.05.006. Epub 2017 May 19.

Abstract

The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Asialoglycoprotein Receptor / genetics*
  • Asialoglycoprotein Receptor / metabolism
  • CRISPR-Cas Systems
  • Cell Differentiation / physiology
  • Cell Line
  • Frameshift Mutation
  • Gene Deletion
  • Gene Knockout Techniques
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • ASGR1 protein, human
  • Asialoglycoprotein Receptor
  • Transcription Factors