Microdissection and microcloning of the long arm of human chromosome 7

R Kaiser; J Weber; K H Grzeschik; J E Edström; A Driesel; S Zengerling; M Buchwald; L C Tsui; K Olek

doi:10.1007/BF00580643

Microdissection and microcloning of the long arm of human chromosome 7

Mol Biol Rep. 1987;12(1):3-6. doi: 10.1007/BF00580643.

Authors

R Kaiser, J Weber, K H Grzeschik, J E Edström, A Driesel, S Zengerling, M Buchwald, L C Tsui, K Olek

PMID: 2888014
DOI: 10.1007/BF00580643

Abstract

DNA-fragments from the region of the long arm of human chromosome 7 to which the CF-locus has been mapped recently were isolated by microdissection and microcloning. We developed a new fixation procedure resulting in inserts of 1.0-7.0 kb in length with a mean value of 2.9 kb. Regional mapping of three clones on 7q was carried out by the use of different hybrid cell lines containing fragments of human chromosome 7.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteriophage lambda / genetics
Chromosomes, Human, Pair 7*
Cloning, Molecular*
DNA / genetics
Escherichia coli / genetics
Humans
Hybrid Cells / ultrastructure
Mice
Polymorphism, Restriction Fragment Length

Substances

DNA