A comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter

A Martin-Gallardo; M Montoya-Zavala; B Kelder; J Taylor; H Chen; F C Leung; J J Kopchick

doi:10.1016/0378-1119(88)90103-5

A comparison of bovine growth-hormone gene expression in mouse L cells directed by the Moloney murine-leukemia virus long terminal repeat, simian virus-40 early promoter or cytomegalovirus immediate-early promoter

Gene. 1988 Oct 15;70(1):51-6. doi: 10.1016/0378-1119(88)90103-5.

Authors

A Martin-Gallardo¹, M Montoya-Zavala, B Kelder, J Taylor, H Chen, F C Leung, J J Kopchick

Affiliation

¹ Department of Zoology and Biomedical Sciences, Edison Animal Biotechnology Center, Athens, OH.

PMID: 2853691
DOI: 10.1016/0378-1119(88)90103-5

Abstract

Three transcriptional regulatory regions including a human cytomegalovirus immediate-early region (CMVIE) promoter, the simian virus-40 early region (SV40E) promoter, and Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR) were ligated to the bovine growth hormone (bGH)-coding gene. Using a bGH transient expression system, the CMVIE and SV40E gene transcriptional regulatory regions were found to be approximately two-fold more efficient than the MoMLV LTR in directing expression of bGH in mouse L cells.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cytomegalovirus / genetics
DNA, Recombinant
Genes, Regulator*
Genetic Vectors
Growth Hormone / genetics*
L Cells
Mice
Moloney murine leukemia virus / genetics
Plasmids
Promoter Regions, Genetic*
Simian virus 40 / genetics
Transfection

Substances

DNA, Recombinant
Growth Hormone