A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons

Beomsue Kim; Suihan Feng; Seong-Wook Yun; Cheryl Leong; Rudrakanta Satapathy; Si Yan Diana Wan; Young-Tae Chang

doi:10.1002/cbic.201600490

A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons

Chembiochem. 2016 Nov 17;17(22):2118-2122. doi: 10.1002/cbic.201600490. Epub 2016 Oct 26.

Authors

Beomsue Kim¹, Suihan Feng², Seong-Wook Yun¹, Cheryl Leong¹, Rudrakanta Satapathy², Si Yan Diana Wan¹, Young-Tae Chang^{2

1}

Affiliations

¹ Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
² Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore.

PMID: 27782351
DOI: 10.1002/cbic.201600490

Abstract

Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2^low cells from heterogeneous cell populations.

Keywords: ABC transporters; Abcg2; CDg13; cell recognition; fluorescent probes; neural stem/progenitor cells.

MeSH terms

ATP Binding Cassette Transporter, Subfamily G, Member 2 / antagonists & inhibitors
ATP Binding Cassette Transporter, Subfamily G, Member 2 / genetics
ATP Binding Cassette Transporter, Subfamily G, Member 2 / metabolism
Animals
Benzamides / chemistry*
Benzamides / metabolism
Biomarkers / metabolism
Brain / cytology
Brain / metabolism
Cell Differentiation
Cells, Cultured
Embryo, Mammalian / cytology
Fluorescent Dyes / chemistry*
Humans
Mice
Microscopy, Fluorescence
Neoplasm Proteins / antagonists & inhibitors
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism
Neural Stem Cells / chemistry
Neural Stem Cells / cytology
Neural Stem Cells / metabolism*
Neurons / cytology
Neurons / metabolism*
Propionates / chemistry
Propionates / metabolism
Quinolines / chemistry
Quinolines / metabolism
RNA Interference
RNA, Small Interfering / metabolism
Xanthenes / chemistry*
Xanthenes / metabolism

Substances

ABCG2 protein, human
ATP Binding Cassette Transporter, Subfamily G, Member 2
Benzamides
Biomarkers
CDg13 compound
Fluorescent Dyes
Neoplasm Proteins
Propionates
Quinolines
RNA, Small Interfering
Xanthenes
verlukast