A new case report of urinary tract infection due to KPC-3-producing klebsiella pneumoniae (ST258) in Spain
Arch Esp Urol. 2016 Sep;69(7):437-40.
[Article in
English,
Spanish]
Affiliations
- 1 Departamento de Microbiología, Facultad de Medicina, Universidad de Granada-ibs. Granada.
- 2 UGC de Medicina Interna. Complejo Hospitalario Universitario de Granada-ibs. Granada.
- 3 Laboratorio de Antibióticos, Servicio de Bacteriología, Centro Nacional de Microbiologia. Majadahonda. Madrid.
- 4 Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada-ibs. Granada. España.
- 5 Departamento de Microbiología, Facultad de Medicina, Universidad de Granada-ibs. Granada. Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada-ibs. Granada. España.
Abstract
Objective:
We describe the characterization of a new isolated in Spain of Klebsiella pneumoniae ST258 producing KPC-3, carbapenems non-susceptible, recovered from a sample of urine from a patient with urinary tract infection and no history of carbapenems exposure.
Methods:
After the isolation, identification of K. pneumoniae was performed by biochemical tests and mass spectrometry. The carbapenems susceptibility testing was performed by microdilution and E-test in cation-adjusted Mueller-Hinton. The study was completed by Rapidec® Carba NP. In order to determine the genetic basis of resistance to carbapenems we used Xpert® Carba-R for carbapenemase type and subtype was subsequently analyzed by amplification by PCR and sequencing.
Result:
We demonstrated by MLST that the strain belonged to the clone of high-risk ST258.
Conclusions:
This is the first characterization, in our media, of a clinical isolated of K. pneumoniae ST258 producing KPC-3 and no history of carbapenems exposure.
MeSH terms
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Aged, 80 and over
-
Bacterial Proteins / biosynthesis
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Female
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Humans
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Klebsiella Infections / microbiology*
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Klebsiella pneumoniae* / enzymology
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Spain
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Urinary Tract Infections / microbiology*
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beta-Lactamases / biosynthesis
Substances
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Bacterial Proteins
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beta-Lactamases
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beta-lactamase KPC-3, Klebsiella pneumoniae