A new case report of urinary tract infection due to KPC-3-producing klebsiella pneumoniae (ST258) in Spain

Arch Esp Urol. 2016 Sep;69(7):437-40.
[Article in English, Spanish]

Abstract

Objective: We describe the characterization of a new isolated in Spain of Klebsiella pneumoniae ST258 producing KPC-3, carbapenems non-susceptible, recovered from a sample of urine from a patient with urinary tract infection and no history of carbapenems exposure.

Methods: After the isolation, identification of K. pneumoniae was performed by biochemical tests and mass spectrometry. The carbapenems susceptibility testing was performed by microdilution and E-test in cation-adjusted Mueller-Hinton. The study was completed by Rapidec® Carba NP. In order to determine the genetic basis of resistance to carbapenems we used Xpert® Carba-R for carbapenemase type and subtype was subsequently analyzed by amplification by PCR and sequencing.

Result: We demonstrated by MLST that the strain belonged to the clone of high-risk ST258.

Conclusions: This is the first characterization, in our media, of a clinical isolated of K. pneumoniae ST258 producing KPC-3 and no history of carbapenems exposure.

Publication types

  • Case Reports

MeSH terms

  • Aged, 80 and over
  • Bacterial Proteins / biosynthesis
  • Female
  • Humans
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae* / enzymology
  • Spain
  • Urinary Tract Infections / microbiology*
  • beta-Lactamases / biosynthesis

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • beta-lactamase KPC-3, Klebsiella pneumoniae