Long Non-Coding RNA ucoo2kmd.1 Regulates CD44-Dependent Cell Growth by Competing for miR-211-3p in Colorectal Cancer

Xiaoli Wu; Xixi He; Shi Li; Xiaoqun Xu; Xiangjian Chen; Hua Zhu

doi:10.1371/journal.pone.0151287

Long Non-Coding RNA ucoo2kmd.1 Regulates CD44-Dependent Cell Growth by Competing for miR-211-3p in Colorectal Cancer

PLoS One. 2016 Mar 14;11(3):e0151287. doi: 10.1371/journal.pone.0151287. eCollection 2016.

Authors

Xiaoli Wu¹, Xixi He¹, Shi Li², Xiaoqun Xu³, Xiangjian Chen⁴, Hua Zhu⁵

Affiliations

¹ Department of gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
² Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
³ Operating room, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
⁴ Department of endoscopic surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
⁵ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.

Abstract

In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs. Long non-coding RNAs (lncRNAs) have been described as the largest subclass of the non-coding transcriptome in human noncoding RNAs. In recent years, lncRNAs have been considered to be the key regulators of tumor behavior. In this study, based on previous research, we investigated the expression and biological role of a newly identified cancer-related lncRNA, lncRNA-uc002kmd.1. We analyzed the relationship between lncRNA-uc002kmd.1 and colorectal cancer (CRC) in a total 45 CRC and paired adjacent, non-tumor tissue samples. We found that lncRNA-uc002kmd.1 expression was usually highly expressed in carcinoma compared with the tissue adjacent to the carcinoma. Through a series of experiments, the results showed that lncRNA-uc002kmd.1 regulates CD44 as a molecular decoy for miR211-3p. Our data indicated that the overexpression of lncRNA-uc002kmd.1 enhanced cell proliferation in CRC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Proliferation
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / pathology*
Demography
Female
Gene Expression Regulation, Neoplastic
HCT116 Cells
Humans
Hyaluronan Receptors / metabolism*
Male
MicroRNAs / genetics*
MicroRNAs / metabolism
Middle Aged
RNA, Long Noncoding / genetics*
RNA, Long Noncoding / metabolism
Up-Regulation
Xenograft Model Antitumor Assays

Substances

Hyaluronan Receptors
MIRN211 microRNA, human
MicroRNAs
RNA, Long Noncoding
long non-coding RNA ucoo2kmd.1, human

Grants and funding

The Project was supported by a grant from Natural Science Foundation of Zhejiang Province of China (LY16H160048). Also this work was supported by a grant from Wenzhou Public Welfare Science and Technology Project (Y20140707), also was supported by a grant from Incubation Project of The First Affiliated Hospital of Wenzhou Medical University (FHY2014009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.