MiR-1303 Regulates Mycobacteria Induced Autophagy by Targeting Atg2B

Kin Yi Au; John C H Pong; Wai Lim Ling; James C B Li

doi:10.1371/journal.pone.0146770

MiR-1303 Regulates Mycobacteria Induced Autophagy by Targeting Atg2B

PLoS One. 2016 Jan 15;11(1):e0146770. doi: 10.1371/journal.pone.0146770. eCollection 2016.

Authors

Kin Yi Au¹, John C H Pong¹, Wai Lim Ling¹, James C B Li¹

Affiliation

¹ Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

Abstract

MicroRNAs are emerging post-transcriptional regulators of gene expressions in both innate immunity and adaptive immunity. In mycobacteria infection, autophagy plays an important role in innate defense mechanism and is tightly regulated by the autophagy-related proteins. Here, we show that Atg2B is involved in the regulation of mycobacteria-induced autophagy. MiR-1303, which function is not defined yet, is found to negatively regulate mycobacteria-induced Atg2B protein production, ultimately down-regulate mycobacteria-induced autophagy. MiR-1303 production is shown to be upregulated during BCG infection and its production is regulated by PI3K and NFκB. It is also demonstrated that miR-1303 targets putative target sites on Atg2B and possibly represses its translation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autophagy / genetics
Autophagy / physiology*
Autophagy-Related Proteins
Bacillus / physiology*
Blotting, Western
Cells, Cultured
Gene Expression Regulation
Humans
Immunohistochemistry
MicroRNAs / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*

Substances

ATG2B protein, human
Autophagy-Related Proteins
MicroRNAs
Vesicular Transport Proteins

Grants and funding

This work was supported by a grant from Health and Medical Research Fund Hong Kong (13121172), http://rfs1.fhb.gov.hk/english/welcome/welcome.html. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.