miR-190a inhibits epithelial-mesenchymal transition of hepatoma cells via targeting the long non-coding RNA treRNA

Xinyu Wang; Yanli Ren; Xinyu Yang; Xiangyu Xiong; Sichong Han; Yunxia Ge; Wenting Pan; Liqing Zhou; Qipeng Yuan; Ming Yang

doi:10.1016/j.febslet.2015.11.024

miR-190a inhibits epithelial-mesenchymal transition of hepatoma cells via targeting the long non-coding RNA treRNA

FEBS Lett. 2015 Dec 21;589(24 Pt B):4079-87. doi: 10.1016/j.febslet.2015.11.024. Epub 2015 Nov 19.

Authors

Xinyu Wang¹, Yanli Ren², Xinyu Yang², Xiangyu Xiong², Sichong Han², Yunxia Ge², Wenting Pan², Liqing Zhou³, Qipeng Yuan², Ming Yang⁴

Affiliations

¹ State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China; Department of Genetics, Harvard Medical School, Boston, MA, USA.
² State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
³ Department of Radiation Oncology, Huaian No. 2 Hospital, Huaian, Jiangsu Province, China.
⁴ State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China. Electronic address: yangm@mail.buct.edu.cn.

PMID: 26608035
DOI: 10.1016/j.febslet.2015.11.024

Abstract

treRNA is a long non-coding RNA (lncRNA) involved in cancer progression. In this study, we show that miR-190a can silence treRNA post-transcriptionally. Suppression of treRNA by miR-190a led to significant changes of mesenchymal-epithelial transition markers and impaired migration and invasion capability of hepatoma cells. TCGA data indicated that miR-190a exhibited lower expression in hepatoma tissues, especially from patients with vascular tumor invasion, compared to normal tissues. Our results reveal the involvement of miR-190a-treRNA axis in hepatoma progression and shed light on lncRNA-based cancer therapies for hepatoma patients at high risk of metastasis.

Keywords: Epithelial-to-mesenchymal transition; Hepatoma; Long non-coding RNA; Translation regulatory long non-coding RNA; miR-190a.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Biomarkers / metabolism
Carcinoma, Hepatocellular / metabolism*
Carcinoma, Hepatocellular / pathology
Cell Line, Tumor
Cell Movement
Down-Regulation
Epithelial-Mesenchymal Transition*
Gene Expression Regulation, Neoplastic
Genes, Reporter
Humans
Liver / metabolism
Liver / pathology
Liver Neoplasms / metabolism*
Liver Neoplasms / pathology
MicroRNAs / metabolism*
Mutation
Neoplasm Invasiveness
RNA / antagonists & inhibitors
RNA / metabolism
RNA Interference*
RNA, Long Noncoding / antagonists & inhibitors*
RNA, Long Noncoding / metabolism
RNA, Neoplasm / metabolism*
RNA, Small Interfering

Substances

Biomarkers
MIRN190 microRNA, human
MicroRNAs
RNA, Long Noncoding
RNA, Neoplasm
RNA, Small Interfering
RNA, recombinant
tre long noncoding RNA, human
RNA