Generation of human iPSC line GRX-MCiPS4F-A2 from adult peripheral blood mononuclear cells (PBMCs) with Spanish genetic background

Sonia Cabrera; Ae-Ri Ji; Lidia Frejo; Veronica Ramos-Mejia; Tamara Romero; Pedro Real; Jose A Lopez-Escamez

doi:10.1016/j.scr.2015.07.002

Generation of human iPSC line GRX-MCiPS4F-A2 from adult peripheral blood mononuclear cells (PBMCs) with Spanish genetic background

Stem Cell Res. 2015 Sep;15(2):337-40. doi: 10.1016/j.scr.2015.07.002. Epub 2015 Jul 23.

Authors

Sonia Cabrera¹, Ae-Ri Ji², Lidia Frejo¹, Veronica Ramos-Mejia³, Tamara Romero³, Pedro Real³, Jose A Lopez-Escamez⁴

Affiliations

¹ Otology & Neurotology Group CTS495, Department of Genomic Medicine, GENYO - Centre for Genomics and Oncological Research - Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016 Spain.
² Otology & Neurotology Group CTS495, Department of Genomic Medicine, GENYO - Centre for Genomics and Oncological Research - Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016 Spain; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, Republic of Korea.
³ Gene regulation, Stem Cells and Development Group, Department of Genomic Oncology, GENYO - Centre for Genomics and Oncological Research - Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016 Spain.
⁴ Otology & Neurotology Group CTS495, Department of Genomic Medicine, GENYO - Centre for Genomics and Oncological Research - Pfizer/University of Granada/Junta de Andalucía, PTS, Granada 18016 Spain; Department of Otolaryngology, University of Granada Hospital, Granada, Spain.

PMID: 26284980
DOI: 10.1016/j.scr.2015.07.002

Abstract

We have generated iPSCs from peripheral blood mononuclear cells (PBMCs) of a healthy man using heat sensitive and non-integrative Sendai virus containing Sox2, Oct3/4, c-Myc and Klf4. Human GRX-MCiPS4F-A2 cell line was established and characterized through this study.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Hispanic or Latino / genetics
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Karyotyping
Kruppel-Like Factor 4
Leukocytes, Mononuclear / cytology*
Male
Proto-Oncogene Proteins c-myc / genetics
Proto-Oncogene Proteins c-myc / metabolism
Teratoma / metabolism
Teratoma / pathology
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Proto-Oncogene Proteins c-myc
Transcription Factors