Human chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1

Kam-Hei So; Suranga P Kodithuwakku; Kottawattage S A Kottawatta; Raymond H W Li; Philip C N Chiu; Annie N Y Cheung; Ernest H Y Ng; William S B Yeung; Kai-Fai Lee

doi:10.1016/j.fertnstert.2015.04.030

Human chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen-activated protein kinase pathway and down-regulation of olfactomedin-1

Fertil Steril. 2015 Aug;104(2):474-82. doi: 10.1016/j.fertnstert.2015.04.030. Epub 2015 May 18.

Authors

Kam-Hei So¹, Suranga P Kodithuwakku², Kottawattage S A Kottawatta³, Raymond H W Li⁴, Philip C N Chiu⁴, Annie N Y Cheung⁵, Ernest H Y Ng⁴, William S B Yeung⁴, Kai-Fai Lee⁶

Affiliations

¹ Department of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China.
² Department of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China; Department of Animal Science, Faculty of Agriculture, The University of Peradeniya, Peradeniya, Sri Lanka.
³ Department of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China; Department of Veterinary Public Health and Pharmacology, Faculty of Veterinary Medicine and Animal Science, The University of Peradeniya, Peradeniya, Sri Lanka.
⁴ Department of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China; Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China; ShenZhen Key Laboratory of Fertility Regulation, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People's Republic of China.
⁵ Department of Pathology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China.
⁶ Department of Obstetrics and Gynaecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China; Centre for Reproduction, Development and Growth, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, People's Republic of China; ShenZhen Key Laboratory of Fertility Regulation, The University of Hong Kong-Shenzhen Hospital, Shenzhen, People's Republic of China. Electronic address: ckflee@hku.hk.

PMID: 25999259
DOI: 10.1016/j.fertnstert.2015.04.030

Abstract

Objective: To study the effect of human chorionic gonadotropin (hCG) on olfactomedin-1 (Olfm1) expression and spheroid attachment in human fallopian tube epithelial cells in vitro.

Design: Experimental study.

Setting: Reproductive biology laboratory.

Patient(s): Healthy nonpregnant women.

Intervention(s): No patient interventions.

Main outcome measure(s): Luteinizing hormone/chorionic gonadotropin receptor (LHCGR) and Olfm1 expression in fallopian tube epithelium cell line (OE-E6/E7 cells). OE-E6/E7 cells treated with hCG, U0126 extracellular signal-regulated kinase (ERK) inhibitor, or XAV939 Wnt/β-catenin inhibitor were analyzed by Western blotting, real-time polymerase chain reaction, and in vitro spheroid attachment assay.

Result(s): Human chorionic gonadotropin increased spheroid attachment on OE-E6/E7 cells through down-regulation of Olfm1 and activation of Wnt and mitogen-activated protein kinase (MAPK) signaling pathways. U0126 down-regulated both MAPK and Wnt/β-catenin signaling pathways and up-regulated Olfm1 expression. XAV939 down-regulated only the Wnt/β-catenin signaling pathway but up-regulated Olfm1 expression.

Conclusion(s): Human chorionic gonadotropin activated both ERK and Wnt/β-catenin signaling pathways and enhanced spheroid attachment on fallopian tube epithelial cells through down-regulation of Olfm1 expression.

Keywords: MAPK signaling; Olfactomedin-1; Wnt-signaling; fallopian tube; tubal ectopic pregnancy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Line
Chorionic Gonadotropin / pharmacology*
Coculture Techniques
Dose-Response Relationship, Drug
Down-Regulation / drug effects
Down-Regulation / physiology
Epithelial Cells / drug effects
Epithelial Cells / metabolism*
Extracellular Matrix Proteins / metabolism*
Fallopian Tubes / cytology
Fallopian Tubes / drug effects
Fallopian Tubes / metabolism*
Female
Glycoproteins / metabolism*
Humans
MAP Kinase Signaling System / drug effects
MAP Kinase Signaling System / physiology*
Middle Aged
Trophoblasts / metabolism*

Substances

Chorionic Gonadotropin
Extracellular Matrix Proteins
Glycoproteins
olfactomedin