Effect of phytic acid etchant on the structural stability of demineralized dentine and dentine bonding

Kalyan Kong; Md Sofiqul Islam; Mohannad Nassar; Noriko Hiraishi; Masayuki Otsuki; Cynthia K Y Yiu; Junji Tagami

doi:10.1016/j.jmbbm.2015.03.027

Effect of phytic acid etchant on the structural stability of demineralized dentine and dentine bonding

J Mech Behav Biomed Mater. 2015 Aug:48:145-152. doi: 10.1016/j.jmbbm.2015.03.027. Epub 2015 Apr 6.

Authors

Kalyan Kong¹, Md Sofiqul Islam², Mohannad Nassar³, Noriko Hiraishi⁴, Masayuki Otsuki⁵, Cynthia K Y Yiu⁶, Junji Tagami⁷

Affiliations

¹ Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Electronic address: kongkalyan@yahoo.com.
² Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Electronic address: soheltmd@gmail.com.
³ Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Electronic address: nassarnar@gmail.com.
⁴ Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Department of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong. Electronic address: hiraope@tmd.ac.jp.
⁵ Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Electronic address: otsuki.ope@tmd.ac.jp.
⁶ Department of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong. Electronic address: ckyyiu@hku.hk.
⁷ Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Electronic address: tagami.ope@tmd.ac.jp.

PMID: 25933170
DOI: 10.1016/j.jmbbm.2015.03.027

Abstract

Objective: This study examined the effect of phytic acid (IP6) in stabilizing the morphology of dentine collagen network and resin-dentine bonding.

Methods: Dentine beams were fully demineralized with 10% phosphoric acid (PA) or 1% IP6 (pH 1.2). PA-demineralized beams were divided into three groups: (a) no further treatment (control), (b) treatment with 5% glutaraldehyde (GA) for 1 h and (c) treatment with 1% IP6 (pH 7) for 1 h. IP6-demineralized beams received no further treatment. The beams were then subjected to ultimate tensile strength (UTS) testing. Dentine micromorphology evaluation was performed using a field-emission scanning electron microscope (FE-SEM). Dentine disks were etched with 35% PA for 15 s or 1% IP6 for 30s. PA-etched dentine disks were divided into three groups as (a), (b) and (c) as for UTS testing, but the treatment with GA or IP6 was done in 1min. For microtensile bond strength (µTBS) testing, flat dentine surfaces etched with PA or IP6 were blot-dried (wet dentine) or air-dried for 10s (dry dentine) and bonded with an etch-and-rinse adhesive followed by composite build-up.

Results: IP6-demineralized dentine showed significantly higher UTS, when compared to PA-demineralized dentine. GA and IP6 significantly improved UTS of PA-demineralized dentine. FE-SEM observation revealed that dentine collagen network was preserved by GA and IP6. No significant difference in µTBS was found between the wet and dry IP6-etched dentine groups.

Conclusion: IP6 etching showed a structural stabilizing effect on demineralized dentine matrix and produced good resin-dentine bonding, regardless of dentine moistness or dryness.

Keywords: Cross-linker; Glutaraldehyde; Microtensile bond strength; Phosphoric acid; Phytic acid; Ultimate tensile strength.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dental Bonding*
Dental Stress Analysis
Dentin / drug effects*
Dentin / physiology
Humans
Phytic Acid / pharmacology*
Resin Cements
Surface Properties
Tensile Strength*
Tooth / drug effects*
Tooth / physiology

Substances

Demineralized Dentin Matrix
Resin Cements
Phytic Acid