Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry

Will Howat; Jodi Miller; Ioannis Gounaris

doi:10.12688/f1000research.5514.2

Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry

F1000Res. 2014 Oct 15:3:244. doi: 10.12688/f1000research.5514.2. eCollection 2014.

Authors

Will Howat¹, Jodi Miller¹, Ioannis Gounaris¹

Affiliation

¹ Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, CB2 0RE, UK.

Abstract

ARID1A is a known suppressor of tumour formation and the Human Protein Atlas antibody HPA005456 has been demonstrated in previous literature to stain tumour tissue by immunohistochemistry (IHC) in formalin-fixed paraffin embedded human tissue and human cell lines. This article details the validation of this antibody for immunohistochemistry of formalin-fixed paraffin embedded murine tissue using a Leica BondMax immunostainer. Using Western blot and IHC on murine wild-type and knockout tissue we have demonstrated that this antibody to ARID1A correctly stains murine tissue by immunohistochemistry.

Keywords: ARID1A; antibody; immunohistochemistry; tissue.

Abstract

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