Assessment of the stability of proanthocyanidins and other phenolic compounds in cranberry syrup after gamma-irradiation treatment and during storage

Food Chem. 2015 May 1:174:392-9. doi: 10.1016/j.foodchem.2014.11.061. Epub 2014 Nov 18.

Abstract

Shelf life of commercial cranberry syrup irradiated with gamma radiation at a rate of 5 kGy and stored for 6 months at 25 °C and 60% relative humidity (RH) and under accelerated stability conditions was investigated. High-performance liquid chromatography coupled to electrospray ionisation quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS) was used to characterise cranberry syrup. Afterwards, these compounds were quantified by HPLC-ESI-QTOF-MS and 4-dimethylaminocinnamaldehyde (DMAC) assay. A significant increase in the content of procyanidin B isomer 1 (from 4.4 to 7.0 μg/ml) and procyanidin A2 (from 83 to 93 μg/ml) was observed after irradiation and compared with the non-irradiated syrup. Procyanidin B isomers and prodelphinidin were stable at 25 °C during the first month of storage, whereas quercetin and some derivatives remained constant for 3 months of storage at this temperature. In short, after gamma-irradiation in dose of 5 kGy, most compounds were highly stable for a month at 25 °C.

Keywords: Cranberry; DMAC; Gamma-irradiation; HPLC; Mass spectrometry; Stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biflavonoids / analysis
  • Catechin / analysis
  • Chromatography, High Pressure Liquid
  • Cinnamates / analysis
  • Dose-Response Relationship, Radiation
  • Food Irradiation*
  • Food Storage*
  • Gamma Rays
  • Limit of Detection
  • Phenols / analysis*
  • Proanthocyanidins / analysis
  • Proanthocyanidins / chemistry*
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry
  • Vaccinium macrocarpon / chemistry*

Substances

  • Biflavonoids
  • Cinnamates
  • Phenols
  • Proanthocyanidins
  • procyanidin A2
  • procyanidin B
  • proanthocyanidin
  • Catechin
  • 4-dimethylaminocinnamaldehyde