Abstract
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
Keywords:
Cas9 protein; Drosophila; RGEN.
Copyright © 2014 Lee et al.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Clustered Regularly Interspaced Short Palindromic Repeats / genetics
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DNA-Binding Proteins / genetics
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Drosophila / genetics
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism
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Genome*
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Germ-Line Mutation
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RNA Editing
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RNA, Guide, CRISPR-Cas Systems
Substances
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DNA-Binding Proteins
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Drosophila Proteins
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e protein, Drosophila