Biodiversity and evolution of Imjin virus and Thottapalayam virus in Crocidurinae shrews in Zhejiang Province, China

Xian-Dan Lin; Run-Hong Zhou; Fei-Neng Fan; Xu-Hua Ying; Xiao-Yu Sun; Wen Wang; Edward C Holmes; Yong-Zhen Zhang

doi:10.1016/j.virusres.2014.05.014

Biodiversity and evolution of Imjin virus and Thottapalayam virus in Crocidurinae shrews in Zhejiang Province, China

Virus Res. 2014 Aug 30:189:114-20. doi: 10.1016/j.virusres.2014.05.014. Epub 2014 May 26.

Authors

Xian-Dan Lin¹, Run-Hong Zhou², Fei-Neng Fan³, Xu-Hua Ying⁴, Xiao-Yu Sun¹, Wen Wang⁵, Edward C Holmes⁶, Yong-Zhen Zhang⁷

Affiliations

¹ Wenzhou Center for Disease Control and Prevention, Wenzhou 325001, Zhejiang Province, China.
² State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206, Beijing, China.
³ Cixi Center for Disease Control and Prevention, Cixi, 315300, Zhejiang Province, China.
⁴ Yuhuan Center for Disease Control and Prevention, Yuhuan, 317600, Zhejiang Province, China.
⁵ State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China.
⁶ State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206, Beijing, China; Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Biological Sciences and Sydney Medical School, The University of Sydney, Sydney, Australia.
⁷ State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China. Electronic address: zhangyongzhen@icdc.cn.

PMID: 24874196
DOI: 10.1016/j.virusres.2014.05.014

Abstract

The recent discovery of numerous hantaviruses in insectivores has provided a new view of hantavirus biodiversity and evolution. To determine the presence and genetic diversity of Imjin virus (MJNV) and Thottapalayam virus (TPMV) in insectivores in Zhejiang Province, China, we captured and performed virus screening of 32 Ussuri white-toothed shrews (Crocidura lasiura) and 105 Asian house shrews (Suncus murinus) in different coastal regions. Hantavirus genome (S, M, and L segments) sequences were successfully recovered from one Ussuri white-toothed shrew and seven Asian house shrews. Phylogenetic analysis revealed that the virus carried by the Ussuri white-toothed shrew was most closely related to MJNV, but with >15% nucleotide sequence difference, suggesting that it represents a new subtype. The hantaviruses carried by Asian house shrews were closely related to the TPMV variants found in the same geographic area, but more distantly related to those sampled in India and Nepal. Additionally, the TPMV sequences obtained in this study, as well as those found previously in this area, could be divided into three lineages reflecting their geographic origins, indicative of largely allopatric evolution. Overall, our data highlights the high genetic diversity of insectivore-borne hantaviruses in China, suggesting that more may be discovered in the future.

Keywords: Evolution; Hantaviruses; Imjin virus; Shrews; Thottapalayam virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biodiversity*
China
Cluster Analysis
Evolution, Molecular*
Molecular Sequence Data
Orthohantavirus / classification*
Orthohantavirus / genetics
Orthohantavirus / isolation & purification*
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Shrews / virology*