Multistep DNA-templated synthesis using a universal template

J Am Chem Soc. 2013 Nov 27;135(47):17727-30. doi: 10.1021/ja409936r. Epub 2013 Nov 18.

Abstract

We report a DNA-templated synthesis method that allows construction of the entire DNA-encoded library with a single DNA template. Taking advantage of deoxyinosine's indiscriminate base-pairing property, we designed a "universal template" that is capable of directing chemical reactions with multiple reactant DNAs with different sequences. In combination with other design features including photocleavable linkers and direct encoding by the reactant DNA, we demonstrated the capabilities of the universal template in library synthesis, target selection, and hit decoding. Our method can be generally and straightforwardly applied to prepare a variety of chemically diverse DNA-encoded libraries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pairing
  • Base Sequence
  • DNA / chemical synthesis*
  • DNA / chemistry
  • Gene Library
  • Molecular Sequence Data

Substances

  • DNA