GmSAL1 hydrolyzes inositol-1,4,5-trisphosphate and regulates stomatal closure in detached leaves and ion compartmentalization in plant cells

Yee-Shan Ku; Nicolas Siu-Chung Koo; Francisca Wing-Yen Li; Man-Wah Li; Hongmei Wang; Sau-Na Tsai; Feng Sun; Boon Leong Lim; Wing-Hung Ko; Hon-Ming Lam

doi:10.1371/journal.pone.0078181

GmSAL1 hydrolyzes inositol-1,4,5-trisphosphate and regulates stomatal closure in detached leaves and ion compartmentalization in plant cells

PLoS One. 2013 Oct 22;8(10):e78181. doi: 10.1371/journal.pone.0078181. eCollection 2013.

Authors

Yee-Shan Ku¹, Nicolas Siu-Chung Koo, Francisca Wing-Yen Li, Man-Wah Li, Hongmei Wang, Sau-Na Tsai, Feng Sun, Boon Leong Lim, Wing-Hung Ko, Hon-Ming Lam

Affiliation

¹ Center for Soybean Research, State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR.

Abstract

Inositol polyphosphatases are important regulators since they control the catabolism of phosphoinositol derivatives, which are often signaling molecules for cellular processes. Here we report on the characterization of one of their members in soybean, GmSAL1. In contrast to the substrate specificity of its Arabidopsis homologues (AtSAL1 and AtSAL2), GmSAL1 only hydrolyzes inositol-1,4,5-trisphosphate (IP3) but not inositol-1,3,4-trisphosphate or inositol-1,4-bisphosphate.The ectopic expression of GmSAL1 in transgenic Arabidopsis thaliana led to a reduction in IP3 signals, which was inferred from the reduction in the cytoplasmic signals of the in vivo biomarker pleckstrin homology domain-green florescent protein fusion protein and the suppression of abscisic acid-induced stomatal closure. At the cellular level, the ectopic expression of GmSAL1 in transgenic BY-2 cells enhanced vacuolar Na(+) compartmentalization and therefore could partially alleviate salinity stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / genetics
Abscisic Acid / metabolism
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Glycine max / enzymology*
Glycine max / genetics
Inositol Phosphates / genetics
Inositol Phosphates / metabolism*
Nucleotidases / genetics
Nucleotidases / metabolism*
Phosphoric Monoester Hydrolases
Plant Stomata / genetics
Plant Stomata / metabolism*
Plants, Genetically Modified / enzymology
Plants, Genetically Modified / genetics
Salinity
Sequence Homology
Signal Transduction / physiology*
Sodium / metabolism
Stress, Physiological / physiology

Substances

Arabidopsis Proteins
Inositol Phosphates
inositol-1,2,4,5-tetrakisphosphate
Abscisic Acid
Sodium
Nucleotidases
Phosphoric Monoester Hydrolases
inositol-1,4-bisphosphate 1-phosphatase

Grants and funding

Sources of funding: Hong Kong Research Grants Council General Research Fund (Grant no. 468409), Lo Kwee-Seong Biomedical Research Fund, and Lee Hysan Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.