Using Drosophila larvae to study epidermal wound closure and inflammation

Sirisha Burra; Yan Wang; Amanda R Brock; Michael J Galko

doi:10.1007/978-1-62703-505-7_26

Using Drosophila larvae to study epidermal wound closure and inflammation

Methods Mol Biol. 2013:1037:449-61. doi: 10.1007/978-1-62703-505-7_26.

Authors

Sirisha Burra¹, Yan Wang, Amanda R Brock, Michael J Galko

Affiliation

¹ Department of Biochemistry and Molecular Biology, The University of Texas Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Abstract

This methods chapter describes two methods for creating epithelial wounds in Drosophila larvae: pinch and puncture wounding. It also covers protocols for visualizing epithelial wounds, either in a dissected whole mount preparation or, using transgenic reporter larvae, in a live whole mount preparation. Finally, useful transgenic lines for live genetic screening of genes required for wound closure or inflammation are described.

MeSH terms

Animals
Disease Models, Animal
Drosophila*
Epidermis / injuries*
Immunohistochemistry / methods
Inflammation / etiology*
Inflammation / pathology*
Larva
Microscopy
Wound Healing*

Grants and funding

R01 GM083031/GM/NIGMS NIH HHS/United States