Nuclear patch-clamp electrophysiology of Ca2+ channels

Cold Spring Harb Protoc. 2013 Sep 1;2013(9):885-91. doi: 10.1101/pdb.prot073064.

Abstract

Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.

MeSH terms

  • Calcium / metabolism*
  • Calcium Channels / physiology*
  • Cations, Divalent / metabolism
  • Cells, Cultured
  • Cytological Techniques / methods*
  • Nuclear Envelope / physiology*
  • Patch-Clamp Techniques / methods*

Substances

  • Calcium Channels
  • Cations, Divalent
  • Calcium