Kinetics and thermodynamics of metal binding to the N-terminus of a human copper transporter, hCTR1

Chem Commun (Camb). 2013 Oct 14;49(80):9134-6. doi: 10.1039/c3cc45360j. Epub 2013 Aug 21.

Abstract

The N-terminus of hCTR1 was demonstrated to bind three Cu(+) ions tightly (log K = 14.92) and reversibly via its Met-rich motifs. Ag(+) binds to the protein with the same stoichiometry but much lower affinities than Cu(+). The protein also coordinates two Cu(2+) ions through its ATCUN motif and His-rich motif with lower affinity. This study provides an insight into the selectivity of the transporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Cation Transport Proteins / chemistry*
  • Cation Transport Proteins / metabolism
  • Copper / chemistry*
  • Copper Transporter 1
  • Humans
  • Ions / chemistry
  • Kinetics
  • Protein Binding
  • Silver / chemistry
  • Thermodynamics

Substances

  • Cation Transport Proteins
  • Copper Transporter 1
  • Ions
  • SLC31A1 protein, human
  • Silver
  • Copper