Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila

Genetics. 2013 Sep;195(1):289-91. doi: 10.1534/genetics.113.153825. Epub 2013 Jul 5.

Abstract

We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

Keywords: CRISPR/Cas9; Drosophila; NHEJ; gRNA; genome modifications.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Drosophila / genetics*
  • Gene Targeting / methods*
  • Genome, Insect*