Modeling calcium-overload mediated necrosis in Drosophila

Kai Liu; Yuhong Li; Lei Liu

doi:10.1007/978-1-62703-383-1_15

Modeling calcium-overload mediated necrosis in Drosophila

Methods Mol Biol. 2013:1004:203-13. doi: 10.1007/978-1-62703-383-1_15.

Authors

Kai Liu¹, Yuhong Li, Lei Liu

Affiliation

¹ State Key Lab of Biomembrane and Membrane Biotechnology, School of Life Sciences, Peking University, Beijing, China.

PMID: 23733579
DOI: 10.1007/978-1-62703-383-1_15

Abstract

Necrotic cell death (necrosis) occurs in many acute-onset diseases. However, our poor understanding of its mechanism has greatly limited medical interventions. Here we describe two methods to establish necrosis models in Drosophila. Our strategy is to overload calcium by expression of leaky cation channels.

MeSH terms

Animals
Animals, Genetically Modified
Calcium / metabolism*
Cell Shape
DNA Fragmentation
Drosophila melanogaster / cytology
Drosophila melanogaster / metabolism*
Drosophila melanogaster / ultrastructure
Genetic Testing
Green Fluorescent Proteins / metabolism
Models, Biological*
Necrosis
Neurons / cytology
Neurons / metabolism

Substances

Green Fluorescent Proteins
Calcium