Abstract
Branchial arches are externally visible tissue bands in the head region of all vertebrate embryos. Although initially formed from similar components, each arch will give rise to different head and neck structures. In a screen designed to characterize the molecular control of branchial arch identity in mouse, we identified Pcp4 as a second branchial arch-specific molecular signature. We further show that the transcription factor Hoxa2 binds to Pcp4 chromatin and regulates Pcp4 expression in the second arch. Hoxa2 is also sufficient to induce Pcp4 expression in anterior first arch cells, which are Pcp4-negative.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Branchial Region / cytology
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Branchial Region / embryology
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Branchial Region / metabolism*
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Cells, Cultured
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Chromatin Immunoprecipitation
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Fluorescent Antibody Technique
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Gene Expression Profiling*
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Gene Expression Regulation, Developmental*
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Homeodomain Proteins / genetics*
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Homeodomain Proteins / metabolism
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Mice
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Mice, Knockout
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Oligonucleotide Array Sequence Analysis
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Protein Binding
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Homeodomain Proteins
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Hoxa2 protein, mouse
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Nerve Tissue Proteins
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Pcp4 protein, mouse