Kif5b controls the localization of myofibril components for their assembly and linkage to the myotendinous junctions

Zai Wang; Ju Cui; Wai Man Wong; Xiuling Li; Wenqian Xue; Raozhou Lin; Jing Wang; Peigang Wang; Julian A Tanner; Kathryn S E Cheah; Wutian Wu; Jian-Dong Huang

doi:10.1242/dev.085969

Kif5b controls the localization of myofibril components for their assembly and linkage to the myotendinous junctions

Development. 2013 Feb 1;140(3):617-26. doi: 10.1242/dev.085969.

Authors

Zai Wang¹, Ju Cui, Wai Man Wong, Xiuling Li, Wenqian Xue, Raozhou Lin, Jing Wang, Peigang Wang, Julian A Tanner, Kathryn S E Cheah, Wutian Wu, Jian-Dong Huang

Affiliation

¹ Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

PMID: 23293293
DOI: 10.1242/dev.085969

Abstract

Controlled delivery of myofibril components to the appropriate sites of assembly is crucial for myofibrillogenesis. Here, we show that kinesin-1 heavy chain Kif5b plays important roles in anterograde transport of α-sarcomeric actin, non-muscle myosin IIB, together with intermediate filament proteins desmin and nestin to the growing tips of the elongating myotubes. Mice with Kif5b conditionally knocked out in myogenic cells showed aggregation of actin filaments and intermediate filament proteins in the differentiating skeletal muscle cells, which further affected myofibril assembly and their linkage to the myotendinous junctions. The expression of Kif5b in mutant myotubes rescued the localization of the affected proteins. Functional mapping of Kif5b revealed a 64-amino acid α-helix domain in the tail region, which directly interacted with desmin and might be responsible for the transportation of these proteins in a complex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton / metabolism
Animals
Cell Differentiation
Desmin / genetics
Desmin / metabolism
Gene Expression Regulation, Developmental
Golgi Apparatus / metabolism
Golgi Apparatus / pathology
Green Fluorescent Proteins / metabolism
Hindlimb / metabolism
Hindlimb / pathology
Intercellular Junctions / metabolism*
Intermediate Filament Proteins / genetics
Intermediate Filament Proteins / metabolism
Kinesins / genetics
Kinesins / metabolism*
Mice
Mice, Knockout
Mitochondria / metabolism
Mitochondria / pathology
Muscle Development*
Muscle, Skeletal / metabolism
Muscular Dystrophy, Animal / metabolism
Muscular Dystrophy, Animal / pathology
Myoblasts, Skeletal / metabolism
Myoblasts, Skeletal / pathology
Myofibrils / genetics
Myofibrils / metabolism*
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Nestin
Nonmuscle Myosin Type IIB / metabolism
Protein Binding
Protein Interaction Mapping
Protein Structure, Tertiary
Protein Transport
Tendons / metabolism*

Substances

Desmin
Intermediate Filament Proteins
Nerve Tissue Proteins
Nes protein, mouse
Nestin
enhanced green fluorescent protein
Green Fluorescent Proteins
Kif5b protein, mouse
Nonmuscle Myosin Type IIB
Kinesins