Expression of MMP-1 and TIMP-1 in irradiated mandibles during distraction osteogenesis

Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Dec;114(6):698-703. doi: 10.1016/j.oooo.2012.01.041. Epub 2012 Aug 15.

Abstract

Objectives: The study aimed to evaluate the expression of MMP-1 and TIMP-1 in irradiated mandibles during distraction osteogenesis.

Study design: Rabbits in the experimental group received preoperative radiation of 9 Gy for 5 fractions. After 1 month, all rabbits underwent osteotomy and distraction osteogenesis with 7 days of latency. Three rabbits in the control and experimental groups were killed at days 7, 12, 18, and 25. Specimens were subjected to immunohistochemical examination and real-time polymerase chain reaction analysis.

Results: At day 7, expression of MMP-1 and TIMP-1 was significantly suppressed in the radiotherapy group in contrast to the control group. At day 12, expression of MMP-1 was significantly higher in the control group. At day 18, expression of MMP-1 and TIMP-1 was significantly higher in the control than in the radiotherapy group.

Conclusions: Radiotherapy changes the expression pattern of MMP-1 and TIMP-1.

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers
  • Humans
  • Immunohistochemistry
  • Mandible / enzymology
  • Mandible / metabolism*
  • Matrix Metalloproteinase 1 / metabolism*
  • Osteogenesis, Distraction*
  • Rabbits
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism*

Substances

  • DNA Primers
  • Tissue Inhibitor of Metalloproteinase-1
  • Matrix Metalloproteinase 1