Abstract
H5N1 influenza viruses, which cause disease in humans, have unusually high pathogenicity. The temporal response of primary human monocyte-derived macrophages infected with highly pathogenic H5N1 and seasonal H1N1 influenza viruses was evaluated using mass spectrometry-based quantitative proteomic profiling. This was done in order to demonstrate significant perturbation of the host proteome upon viral infection, as early as 1 hour after infection. This early host response distinguished H5N1 infection from H1N1 infection, the latter inducing less of a response. The most pronounced effect was observed on the translational machinery, suggesting that H5N1 might gain advantage in replication by using the cell protein synthesis machinery early in the infection.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Host-Pathogen Interactions
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Humans
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Influenza A Virus, H1N1 Subtype / immunology
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Influenza A Virus, H1N1 Subtype / metabolism
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Influenza A Virus, H1N1 Subtype / pathogenicity
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Influenza A Virus, H1N1 Subtype / physiology*
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Influenza A Virus, H5N1 Subtype / immunology
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Influenza A Virus, H5N1 Subtype / metabolism
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Influenza A Virus, H5N1 Subtype / pathogenicity
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Influenza A Virus, H5N1 Subtype / physiology*
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Influenza, Human / immunology
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Influenza, Human / metabolism
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Influenza, Human / virology*
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / immunology
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Macrophages / cytology
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Macrophages / immunology
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Macrophages / virology*
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Principal Component Analysis
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Proteomics / methods*
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Tandem Mass Spectrometry