Abstract
Cytotrophoblasts are the key trophoblast cells which differentiate into different trophoblast lineages. In this report, glycodelin-A action on fusion of a cytotrophoblast-like cell line (BeWo) was investigated. It significantly reduced the spontaneous fusion of BeWo cells. The treatment enhanced the invasion and extracellular-signal regulated kinases activation of BeWo cells. The mRNA expression of syncytialization markers, human chorionic gonadotrophin and glial cells missing homolog 1 were suppressed upon glycodelin-A treatment. The data suggest a possible function of glycodelin-A in mediating cytotrophoblast differentiation.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoembryonic Antigen / genetics
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Cell Differentiation / physiology
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Cell Fusion
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Cell Line, Tumor
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Choriocarcinoma
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Chorionic Gonadotropin, beta Subunit, Human / genetics
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Enzyme Activation / drug effects
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Extracellular Signal-Regulated MAP Kinases / analysis
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Female
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Gene Expression / drug effects
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Glycodelin
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Glycoproteins / pharmacology*
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Glycoproteins / physiology
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Humans
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Pregnancy Proteins / pharmacology*
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Pregnancy Proteins / physiology
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Trophoblasts / drug effects
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Trophoblasts / physiology*
Substances
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CEACAM3 protein, human
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Carcinoembryonic Antigen
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Chorionic Gonadotropin, beta Subunit, Human
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Glycodelin
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Glycoproteins
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PAEP protein, human
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Pregnancy Proteins
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Extracellular Signal-Regulated MAP Kinases