Peptidoglycan assembly machines: the biochemical evidence

Microb Drug Resist. 2012 Jun;18(3):256-60. doi: 10.1089/mdr.2011.0236. Epub 2012 Mar 20.

Abstract

To make progress in understanding peptidoglycan metabolism, we will reconstitute in vitro the assembly process and the molecular machineries that carry out this formidable task. We review here the reports of isolation of complexes comprising penicillin-binding proteins (PBPs), the enzymes that synthesize the peptidoglycan from its lipid-linked precursor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Wall / chemistry
  • Cell Wall / genetics
  • Cell Wall / metabolism*
  • Chromatography, Affinity
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Glycosyltransferases / genetics
  • Glycosyltransferases / metabolism
  • Immunoprecipitation
  • Muramoylpentapeptide Carboxypeptidase / genetics
  • Muramoylpentapeptide Carboxypeptidase / metabolism
  • Penicillin-Binding Proteins / genetics
  • Penicillin-Binding Proteins / metabolism
  • Peptidoglycan / biosynthesis*
  • Staining and Labeling

Substances

  • Carrier Proteins
  • Penicillin-Binding Proteins
  • Peptidoglycan
  • Glycosyltransferases
  • Muramoylpentapeptide Carboxypeptidase