Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films

C K M Ng; E Y W Chong; V A L Roy; K M C Cheung; K W K Yeung; K N Yu

doi:10.1016/j.apradiso.2011.11.019

Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films

Appl Radiat Isot. 2012 Jul;70(7):1432-5. doi: 10.1016/j.apradiso.2011.11.019. Epub 2011 Nov 18.

Authors

C K M Ng¹, E Y W Chong, V A L Roy, K M C Cheung, K W K Yeung, K N Yu

Affiliation

¹ Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong.

PMID: 22130471
DOI: 10.1016/j.apradiso.2011.11.019

Abstract

We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on such a micropillar substrate had significantly larger percentage of cells entering S-phase, attached cell numbers and cell spreading areas.