Possible modulation of release of atrial natriuretic factor by endothelium-derived relaxing factor

Am J Physiol. 1990 Sep;259(3 Pt 2):H982-6. doi: 10.1152/ajpheart.1990.259.3.H982.

Abstract

The aim of the present study was to investigate the influence of inhibitors of endothelium-dependent relaxing factor on the release of atrial natriuretic factor from isolated rat atria. Electrical stimulation of the tissue produced a frequency-dependent increase in secretion of atriopeptide from basal levels. Saponin (0.3 mg/ml for 45 min) augmented the basal release of the peptide. Methylene blue (10(-5)M), oxyhemoglobin (10(-6)M), or hydroquinone (10(-5)M), agents that inhibit the effects of endothelium-derived relaxing factor by different mechanisms, caused an increase in the basal secretion of atrial natriuretic factor. Blockade of cyclooxygenase with indomethacin (10(-5)M) did not alter the release of atriopeptide, suggesting that it is not controlled by endogenous prostaglandins. These results would be explained if the inhibitors tested were to interfere with a factor released continuously from either endocardial cells or endothelial cells of the atrial blood vessels and having the same nature as endothelium-derived relaxing factor. Such release may exert a tonic negative modulatory influence on the secretion of atrial natriuretic factor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Atrial Natriuretic Factor / metabolism*
  • Electric Stimulation
  • Heart Atria
  • Hydroquinones / pharmacology
  • In Vitro Techniques
  • Indomethacin / pharmacology
  • Male
  • Methylene Blue / pharmacology
  • Myocardium / metabolism
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / physiology*
  • Oxyhemoglobins / pharmacology
  • Rats
  • Rats, Inbred Strains

Substances

  • Hydroquinones
  • Oxyhemoglobins
  • Nitric Oxide
  • Atrial Natriuretic Factor
  • Methylene Blue
  • hydroquinone
  • Indomethacin