Bone regeneration in the presence of a synthetic hydroxyapatite/silica oxide-based and a xenogenic hydroxyapatite-based bone substitute material

Clin Oral Implants Res. 2011 May;22(5):506-11. doi: 10.1111/j.1600-0501.2010.02039.x. Epub 2010 Dec 2.

Abstract

Objectives: A comparison of synthetic hydroxyapatite/silica oxide, xenogenic hydroxyapatite-based bone substitute materials with empty control sites in terms of bone regeneration enhancement in a rabbit calvarial four non-critical-sized defect model.

Methods: In each of six rabbits, four bicortical calvarial bone defects were generated. The following four treatment modalities were randomly allocated: (1) empty control site, (2) synthetic hydroxyapatite/silica oxide-based (HA/SiO) test granules, (3) xenogenic hydroxyapatite -based granules, (4) synthetic hydroxyapatite/silica oxide -based (HA/SiO) test two granules. The results of the latter granules have not been reported due to their size being three times bigger than the other two granule types. After 4 weeks, the animals were sacrificed and un-decalcified sections were obtained for histological analyses. For statistical analysis, the Kruskal-Wallis test was applied (P<0.05).

Results: Histomorphometric analysis showed an average area fraction of newly formed bone of 12.32±10.36% for the empty control, 17.47±6.42% for the xenogenic hydroxyapatite -based granules group, and 21.2±5.32% for the group treated with synthetic hydroxyapatite/silica oxide -based granules. Based on the middle section, newly formed bone bridged the defect to 38.33±37.55% in the empty control group, 54.33±22.12% in the xenogenic hydroxyapatite -based granules group, and to 79±13.31% in the synthetic hydroxyapatite/silica oxide -based granules group. The bone-to-bone substitute contact was 46.38±18.98% for the xenogenic and 59.86±14.92% for the synthetic hydroxyapatite/silica oxide-based granules group. No significant difference in terms of bone formation and defect bridging could be detected between the two bone substitute materials or the empty defect.

Conclusion: There is evidence that the synthetic hydroxyapatite/silica oxide granules provide comparable results with a standard xenogenic bovine mineral in terms of bone formation and defect bridging in non-critical size defects.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Azo Compounds
  • Bone Diseases / diagnostic imaging
  • Bone Diseases / surgery
  • Bone Matrix / transplantation*
  • Bone Regeneration / physiology*
  • Bone Substitutes / therapeutic use*
  • Cattle
  • Coloring Agents
  • Drug Combinations
  • Durapatite / therapeutic use*
  • Eosine Yellowish-(YS)
  • Frontal Bone / diagnostic imaging
  • Frontal Bone / surgery
  • Methyl Green
  • Minerals / therapeutic use*
  • Nanoparticles / therapeutic use
  • Osteogenesis / physiology
  • Parietal Bone / diagnostic imaging
  • Parietal Bone / surgery
  • Porosity
  • Rabbits
  • Radiography
  • Random Allocation
  • Silicon Dioxide / therapeutic use*
  • Transplantation, Heterologous

Substances

  • Azo Compounds
  • Bio-Oss
  • Bone Substitutes
  • Coloring Agents
  • Drug Combinations
  • Minerals
  • NanoBone
  • trichrome stain
  • Silicon Dioxide
  • Methyl Green
  • Durapatite
  • Eosine Yellowish-(YS)