Expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisor

Liwen Li; Hyuk-Jae Kwon; Hidemitsu Harada; Hayato Ohshima; Sung-Won Cho; Han-Sung Jung

doi:10.1016/j.gep.2010.11.001

Expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisor

Gene Expr Patterns. 2011 Mar-Apr;11(3-4):163-70. doi: 10.1016/j.gep.2010.11.001. Epub 2010 Nov 10.

Authors

Liwen Li¹, Hyuk-Jae Kwon, Hidemitsu Harada, Hayato Ohshima, Sung-Won Cho, Han-Sung Jung

Affiliation

¹ Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korean 21 Project, Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, South Korea.

PMID: 21073982
DOI: 10.1016/j.gep.2010.11.001

Abstract

Recent studies have demonstrated the existence of dental stem cells in the continuously growing tooth. However, much remains to be learned about the complex mechanism involving stem cells during tooth development. We determined the expression patterns of four stem cell markers ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisors between embryonic day (E) 11 and postnatal day (PN) 20. ABCG2 was localized strongly in the perivascular region of the incisor mesenchyme from E11 to PN20, and in the odontoblasts from E18 to PN20. Bmi-1 was expressed in both the dental epithelium and mesenchyme from E11 to E14. The expression of Bmi-1 was noticeably reduced at E16, and was restricted to the apical bud from E16 to PN20. Oct-3/4 was localized in the nucleus of the cells in the superficial layer and stellate reticulum within the dental epithelium from E11 to E14 and in the apical bud from E16 to PN20. Meanwhile, once the ameloblasts and odontoblasts began to appear at E16, they expressed Oct-3/4 in the cytoplasm. Yap was expressed in most of the basal cells of the incisor dental epithelium from E11 to E14, but was expressed mainly in the transit-amplifying (TA) cells within the basal cell layer from E16 to PN20. The unique and overlapping expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap suggest the independent and interactive functions of the four stem cell markers in the developing mouse incisor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP-Binding Cassette Transporters / genetics*
ATP-Binding Cassette Transporters / metabolism
Adaptor Proteins, Signal Transducing / genetics*
Adaptor Proteins, Signal Transducing / metabolism
Animals
Antigens, Differentiation / metabolism
Cell Cycle Proteins
Dental Papilla / embryology
Dental Papilla / growth & development
Dental Papilla / metabolism
Embryonic Stem Cells / metabolism
Epithelium / embryology
Epithelium / growth & development
Epithelium / metabolism
Female
Gene Expression Regulation, Developmental
Incisor / embryology*
Incisor / growth & development*
Incisor / metabolism
Mice
Mice, Inbred ICR
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Octamer Transcription Factor-3 / genetics*
Octamer Transcription Factor-3 / metabolism
Phosphoproteins / genetics*
Phosphoproteins / metabolism
Polycomb Repressive Complex 1
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins / metabolism
Repressor Proteins / genetics*
Repressor Proteins / metabolism
YAP-Signaling Proteins

Substances

ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP-Binding Cassette Transporters
Abcg2 protein, mouse
Adaptor Proteins, Signal Transducing
Antigens, Differentiation
Bmi1 protein, mouse
Cell Cycle Proteins
Nuclear Proteins
Octamer Transcription Factor-3
Phosphoproteins
Proto-Oncogene Proteins
Repressor Proteins
YAP-Signaling Proteins
Yap1 protein, mouse
Polycomb Repressive Complex 1