Effect of platelet-rich plasma on a rabbit model of nicotine-compromised bone healing

J Oral Maxillofac Surg. 2011 Jan;69(1):28-35. doi: 10.1016/j.joms.2010.05.007. Epub 2010 Oct 25.

Abstract

Purpose: This study aimed to evaluate the effects of platelet-rich plasma (PRP) on nicotine-compromised bone healing.

Materials and methods: Fifteen adult New Zealand white rabbits were implanted with 1.5-g time-release nicotine pellets. Bilateral mandibular distraction osteogenesis was then performed. Autologous PRP was injected into 1 side of the distraction regenerate, whereas physiologic saline solution was injected into the contralateral side as a control. Five rabbits were killed on day 5 of active distraction, on day 11 of active distraction, and in week 2 of consolidation, respectively.

Results: In the PRP the platelet enrichment was 14.63 ± 3.081-fold of that in whole blood. Plain radiography and micro-computed tomography assessment showed no significant difference between the PRP injection and control sides. Histologic examination showed more disorganized distraction tissue on the PRP injection side.

Conclusions: PRP injection at an early stage of active distraction does not significantly enhance bone healing in the nicotine-compromised rabbit model of mandibular lengthening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Regeneration / drug effects
  • Connective Tissue / drug effects
  • Connective Tissue / pathology
  • Delayed-Action Preparations
  • Disease Models, Animal
  • Mandible / diagnostic imaging
  • Mandible / drug effects
  • Mandible / surgery*
  • Nicotine / adverse effects*
  • Nicotinic Agonists / adverse effects*
  • Osteogenesis / drug effects
  • Osteogenesis, Distraction / methods
  • Osteotomy / methods
  • Platelet Count
  • Platelet-Rich Plasma / physiology*
  • Rabbits
  • Time Factors
  • Transforming Growth Factor beta1 / analysis
  • Wound Healing / drug effects
  • X-Ray Microtomography

Substances

  • Delayed-Action Preparations
  • Nicotinic Agonists
  • Transforming Growth Factor beta1
  • Nicotine