LIM domain protein FHL1B interacts with PP2A catalytic β subunit--a novel cell cycle regulatory pathway

FEBS Lett. 2010 Nov 19;584(22):4511-6. doi: 10.1016/j.febslet.2010.10.022. Epub 2010 Oct 20.

Abstract

Four-and-a-half LIM domain protein 1B (FHL1B) is an alternatively-spliced isoform of FHL1. In this study, FHL1B was demonstrated to interact with the β catalytic subunit (Cβ) of a type 2A protein phosphatase (PP2A) by yeast two-hybrid screening. Domain studies using a small-scale yeast two-hybrid interaction assay revealed the mediation of protein-protein interaction by FHL1B's C-terminus. Interaction between FHL1B and PP2A was further verified by co-immunoprecipitation. FHL1B was also shown to shuttle between nucleus and cytoplasm at different phases of the cell cycle. These data suggest that the FHL1B/PP2A(Cβ) interaction may illustrate a novel cell-cycle regulatory pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocatalysis
  • Catalytic Domain*
  • Cell Cycle*
  • Cell Line, Tumor
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Intracellular Space / metabolism
  • LIM Domain Proteins
  • Mice
  • Muscle Proteins / genetics
  • Muscle Proteins / metabolism*
  • Protein Binding
  • Protein Phosphatase 2 / chemistry*
  • Protein Phosphatase 2 / metabolism*
  • Protein Transport
  • Reproducibility of Results
  • Sequence Deletion
  • Substrate Specificity

Substances

  • FHL1 protein, human
  • Intracellular Signaling Peptides and Proteins
  • LIM Domain Proteins
  • Muscle Proteins
  • Protein Phosphatase 2