Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells

Sei-ichi Sato; Hiroki Inoue; Takeshi Kogure; Mitsuo Tagaya; Katsuko Tani

doi:10.1016/j.febslet.2010.09.047

Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells

FEBS Lett. 2010 Nov 5;584(21):4389-95. doi: 10.1016/j.febslet.2010.09.047. Epub 2010 Oct 8.

Authors

Sei-ichi Sato¹, Hiroki Inoue, Takeshi Kogure, Mitsuo Tagaya, Katsuko Tani

Affiliation

¹ School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.

PMID: 20932832
DOI: 10.1016/j.febslet.2010.09.047

Abstract

Mammals have three members of the intracellular phospholipase A(1) protein family (phosphatidic acid preferring-phospholipase A(1), p125, and KIAA0725p). In this study, we showed that KIAA0725p is localized in the Golgi, and is rapidly cycled between the Golgi and cytosol. Catalytic activity is important for targeting of KIAA0725p to Golgi membranes. RNA interference experiments suggested that KIAA0725p contributes to efficient membrane trafficking from the Golgi apparatus to the plasma membrane, but is not involved in brefeldin A-induced Golgi-to-endoplasmic reticulum retrograde transport.

MeSH terms

Animals
Biocatalysis
Brefeldin A / pharmacology
Cell Membrane / metabolism*
Cytosol / metabolism
Endoplasmic Reticulum / drug effects
Endoplasmic Reticulum / metabolism
Gene Knockdown Techniques
Golgi Apparatus / metabolism*
HeLa Cells
Humans
N-Acetyllactosamine Synthase / metabolism
Phospholipases A1 / deficiency
Phospholipases A1 / genetics
Phospholipases A1 / metabolism*
Protein Transport / drug effects

Substances

Brefeldin A
N-Acetyllactosamine Synthase
Phospholipases A1