Evaluation of in-house and commercial genotyping assays for molecular typing of hepatitis C virus in Hong Kong

Br J Biomed Sci. 2010;67(2):82-5. doi: 10.1080/09674845.2010.11730296.

Abstract

This study aims to evaluate genotyping assays for hepatitis C virus (HCV). An in-house nucleic acid sequencing method is performed in parallel with the Roche Linear Array HCV genotyping test on 73 HCV-positive (66 clinical samples and seven proficiency testing quality control samples) and 12 HCV-negative samples (11 clinical samples and one proficiency testing sample). The performance of the in-house method was comparable with that of the Roche assay (concordance rate: 89.4%). Discordant results included four mixed infections missed by the in-house method, two false-negatives with the Roche assay, and three discrepant results. The in-house method exhibited a higher resolution (subtype vs. genotype level) at a lower running cost (25% of the commercial assay). The in-house method was also used to genotype 375 HCV clinical isolates to determine the genotypic distribution of HCV in Hong Kong between 2005 and 2008. A total of 441 (52.8%) clinical isolates proved to be genotype 1, which shows a poorer response to interferon therapy. Genotype 6 was the next most common (32.0%). Prevalence of genotypes 2 and 3 was 7.7% and 6.6%, respectively, and prevalence of genotypes 4 and 5 was 0.9% and 0%, respectively. Although the in-house nucleic acid sequencing method failed to detect a few cases of mixed HCV infection, its high resolution and low running cost make it suitable for surveillance and outbreak investigation.

Publication types

  • Evaluation Study

MeSH terms

  • Genotype
  • Hepacivirus / classification
  • Hepacivirus / genetics*
  • Hepacivirus / isolation & purification
  • Hepatitis C / genetics*
  • Hong Kong
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Predictive Value of Tests
  • Reagent Kits, Diagnostic
  • Sequence Analysis, DNA / methods*

Substances

  • Reagent Kits, Diagnostic