Enhanced surface colonization by Escherichia coli O157:H7 in biofilms formed by an Acinetobacter calcoaceticus isolate from meat-processing environments

Appl Environ Microbiol. 2010 Jul;76(13):4557-9. doi: 10.1128/AEM.02707-09. Epub 2010 May 7.

Abstract

A meat factory commensal bacterium, Acinetobacter calcoaceticus, affected the spatial distribution of Escherichia coli O157:H7 surface colonization. The biovolume of E. coli O157:H7 was 400-fold higher (1.2 x 10(6) microm(3)) in a dynamic cocultured biofilm than in a monoculture (3.0 x 10(3) microm(3)), and E. coli O157:H7 colonized spaces between A. calcoaceticus cell clusters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter calcoaceticus / growth & development*
  • Acinetobacter calcoaceticus / ultrastructure
  • Biofilms / growth & development*
  • Coculture Techniques
  • Colony Count, Microbial
  • Escherichia coli O157 / genetics
  • Escherichia coli O157 / growth & development*
  • Escherichia coli O157 / ultrastructure
  • Food Contamination
  • Food-Processing Industry*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Meat* / microbiology
  • Microscopy, Confocal

Substances

  • Green Fluorescent Proteins