Down-regulated genes in mouse dental papillae and pulp

H Sasaki; T Muramatsu; H-J Kwon; H Yamamoto; S Hashimoto; H-S Jung; M Shimono

doi:10.1177/0022034510366844

Down-regulated genes in mouse dental papillae and pulp

J Dent Res. 2010 Jul;89(7):679-83. doi: 10.1177/0022034510366844. Epub 2010 May 6.

Authors

H Sasaki¹, T Muramatsu, H-J Kwon, H Yamamoto, S Hashimoto, H-S Jung, M Shimono

Affiliation

¹ Oral Health Science Center HRC7, Tokyo Dental College, Japan.

PMID: 20448247
DOI: 10.1177/0022034510366844

Abstract

Important factors involved in odontogenesis in mouse dental papillae disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ADAM Proteins / analysis
ADAM Proteins / genetics
ADAMTS4 Protein
Aldehyde Dehydrogenase / analysis
Aldehyde Dehydrogenase / genetics
Aldehyde Dehydrogenase 1 Family
Animals
Cell Death / genetics
Dental Papilla / cytology*
Dental Papilla / embryology
Dental Pulp / cytology*
Dental Pulp / embryology
Down-Regulation / genetics*
Epithelial Cells / cytology
Gene Expression Regulation, Developmental / genetics
In Situ Hybridization
Lymphoid Enhancer-Binding Factor 1 / analysis
Lymphoid Enhancer-Binding Factor 1 / genetics
Mice
Mice, Inbred ICR
Odontoblasts / cytology
Odontogenesis / genetics*
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Procollagen N-Endopeptidase / analysis
Procollagen N-Endopeptidase / genetics
Retinal Dehydrogenase
Tooth Calcification / genetics
Tooth Germ / cytology
Tooth Germ / embryology

Substances

Lef1 protein, mouse
Lymphoid Enhancer-Binding Factor 1
Aldehyde Dehydrogenase 1 Family
Aldehyde Dehydrogenase
Aldh1a2 protein, mouse
Retinal Dehydrogenase
ADAM Proteins
Procollagen N-Endopeptidase
ADAMTS4 Protein
Adamts4 protein, mouse