Cutting edge: granulocyte-macrophage colony-stimulating factor is the major CD8+ T cell-derived licensing factor for dendritic cell activation

Lin Min; Siti Aminah Bte Mohammad Isa; Wang Shuai; Cher Boon Piang; Fam Wee Nih; Masayo Kotaka; Christiane Ruedl

doi:10.4049/jimmunol.0903873

Cutting edge: granulocyte-macrophage colony-stimulating factor is the major CD8+ T cell-derived licensing factor for dendritic cell activation

J Immunol. 2010 May 1;184(9):4625-9. doi: 10.4049/jimmunol.0903873. Epub 2010 Mar 31.

Authors

Lin Min¹, Siti Aminah Bte Mohammad Isa, Wang Shuai, Cher Boon Piang, Fam Wee Nih, Masayo Kotaka, Christiane Ruedl

Affiliation

¹ School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.

PMID: 20357255
DOI: 10.4049/jimmunol.0903873

Abstract

During priming, CD8(+) T lymphocytes can induce robust maturation of dendritic cells (DCs) in a CD40-independent manner by secreting licensing factor(s). In this study, we isolate this so-far elusive licensing factor and identify it, surprisingly, as GM-CSF. This provides a new face for an old factor with a well-known supporting role in DC development and recruitment. Signaling through the GM-CSFR in ex vivo-purified DCs upregulated the expression of costimulatory molecules more efficiently than did any tested TLR agonist and provided a positive feedback loop in the stimulation of CD8(+) T cell proliferation. Combined with a variety of microbial stimuli, GM-CSF supports the formation of potent "effector" DCs capable of secreting a variety of proinflammatory cytokines that guide the differentiation of T cells during the immune response.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD8-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / metabolism*
Cell Communication / immunology
Cell Differentiation / immunology*
Cell Proliferation
Cells, Cultured
Coculture Techniques
Cytokines / metabolism
Cytokines / physiology
Dendritic Cells / immunology*
Dendritic Cells / metabolism*
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
Granulocyte-Macrophage Colony-Stimulating Factor / physiology*
Inflammation Mediators / metabolism
Inflammation Mediators / physiology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / deficiency
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / genetics
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
TCF Transcription Factors / metabolism
TCF Transcription Factors / physiology
Up-Regulation / immunology

Substances

Cytokines
Inflammation Mediators
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
TCF Transcription Factors
Granulocyte-Macrophage Colony-Stimulating Factor