The mutations of Th1 cell-specific T-box transcription factor may be associated with a predominant Th2 phenotype in gastric cancers

P Yang; G Qiu; S Wang; Z Su; J Chen; S Wang; F Kong; L Lu; T Ezaki; H Xu

doi:10.1111/j.1744-313X.2010.00899.x

The mutations of Th1 cell-specific T-box transcription factor may be associated with a predominant Th2 phenotype in gastric cancers

Int J Immunogenet. 2010 Apr;37(2):111-5. doi: 10.1111/j.1744-313X.2010.00899.x. Epub 2010 Feb 19.

Authors

P Yang¹, G Qiu, S Wang, Z Su, J Chen, S Wang, F Kong, L Lu, T Ezaki, H Xu

Affiliation

¹ Department of Immunology, Institute of Clinical Laboratory Medicine, Jiangsu University, Zhenjiang, China.

PMID: 20193034
DOI: 10.1111/j.1744-313X.2010.00899.x

Abstract

Gastric cancer is a serious public health cancer and causes nearly 1 million deaths a year worldwide. Th1 cells play critical roles in orchestrating the adaptive immune responses against gastric cancer. T-bet, a member of the T-box family of transcription factors, is the Th1 master regulator and up-regulated during Th1 differentiation. Polymorphisms have also been shown to exist in T-bet. Some reports indicated that some tumours were associated with the drift of Th1 and Th2. In the present work, we investigated the drift of Th1/Th2 by detecting the expression levels of T-bet, IFN-gamma, IL-4, and GATA-3 in peripheral blood mononuclear cell of gastric cancer patients by real-time PCR, explored the relationship between the polymorphism of T-bet gene and drift of Th1/Th2 by gene sequence, western blot, and gene transfection. Our results indicated that a predominant Th2 phenotype was existence. T-bet gene mutations may be associated with Th2-dominated condition in gastric cancers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Blotting, Western
Cell Line
Cell Line, Tumor
Female
GATA3 Transcription Factor / genetics
GATA3 Transcription Factor / metabolism
Gene Expression Profiling
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Interferon-gamma / genetics
Interferon-gamma / metabolism
Interleukin-4 / genetics
Interleukin-4 / metabolism
Leukocytes, Mononuclear / metabolism
Male
Microscopy, Fluorescence
Middle Aged
Mutation*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / genetics*
Stomach Neoplasms / metabolism
T-Box Domain Proteins / genetics*
T-Box Domain Proteins / metabolism
Th1 Cells / metabolism*
Th2 Cells / metabolism*

Substances

GATA3 Transcription Factor
Recombinant Fusion Proteins
T-Box Domain Proteins
T-box transcription factor TBX21
Green Fluorescent Proteins
Interleukin-4
Interferon-gamma