A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009

J Virol Methods. 2010 Jul;167(1):100-2. doi: 10.1016/j.jviromet.2010.02.001. Epub 2010 Feb 6.

Abstract

A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88% for both human specimens and avian specimens. The corresponding specificity was 99.3% for human specimens and 96.5% for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Influenza A virus / classification*
  • Influenza A virus / isolation & purification*
  • Influenza in Birds / diagnosis*
  • Influenza in Birds / virology
  • Influenza, Human / diagnosis*
  • Influenza, Human / virology
  • Poultry Diseases / diagnosis*
  • Poultry Diseases / virology
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Virology / methods*

Substances

  • Reagent Kits, Diagnostic