Resin-dentine interfacial morphology following CPP-ACP treatment

J Dent. 2010 Feb;38(2):96-105. doi: 10.1016/j.jdent.2009.09.007. Epub 2009 Sep 23.

Abstract

Objectives: To evaluate the quality of bonded resin-dentine interfaces produced by two self-etching primer adhesives after casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) treatment, in comparison to untreated dentine.

Methods: Thirty-four adult molar teeth were sectioned to obtain dentine with tubule orientations parallel/oblique or perpendicular to the surface. The specimens were divided into 'smear layer' (1A, 1B) and 'no smear layer' groups, after treatment with 15% EDTA (2A, 2B). The specimens were then left either untreated (1A, 2A) or treated (1B, 2B) with CPP-ACP paste (Tooth Mousse, GC Corp.) for 60 min x 7 days. Each treatment group was divided into three subgroups and specimens etched/conditioned (no conditioning; 30-40% phosphoric acid (H(3)PO(4)); 20% polyacrylic acid) and bonded with either a 2-step self-etching primer adhesive (Clearfil SE Bond, Kuraray Medical) or an 'all-in-one' adhesive (G-Bond, GC Corp.) and a hybrid resin composite. After 24-h water storage, the bonded specimens were sectioned, polished up to 4000-grit abrasive silicon carbide paper and 0.25-microm diamond paste, prepared for FE-SEM using the acid-base technique, critical point-dried, gold-coated and examined. Bonded and fractured dentine interfaces were also prepared and examined.

Results: The 2-step adhesive produced a similar appearance of bonded resin-dentine interface irrespective of smear layer group, treatment or etching/conditioning. After polyacrylic acid conditioning, the 'all-in-one' adhesive exhibited more areas with bond failures. The bond failures were within the hybrid layer and more pronounced following CPP-ACP treatment.

Conclusion: The quality of the bonded resin-dentine interface produced after CPP-ACP treatment may depend on the adhesive system used.

Publication types

  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Etching, Dental / methods
  • Acrylic Resins / chemistry
  • Adhesiveness
  • Adult
  • Carbon Compounds, Inorganic / chemistry
  • Cariostatic Agents / chemistry*
  • Caseins / chemistry*
  • Chelating Agents / chemistry
  • Composite Resins / chemistry
  • Dental Bonding*
  • Dental Polishing / methods
  • Dentin / ultrastructure*
  • Dentin-Bonding Agents / chemistry
  • Diamond / chemistry
  • Edetic Acid / chemistry
  • Humans
  • Materials Testing
  • Methacrylates / chemistry
  • Microscopy, Electron, Scanning
  • Phosphoric Acids / chemistry
  • Resin Cements / chemistry*
  • Silicon Compounds / chemistry
  • Smear Layer
  • Surface Properties
  • Time Factors
  • Water / chemistry

Substances

  • Acrylic Resins
  • Carbon Compounds, Inorganic
  • Cariostatic Agents
  • Caseins
  • Chelating Agents
  • Clearfil SE Bond
  • Composite Resins
  • Dentin-Bonding Agents
  • G-Bond
  • Methacrylates
  • Phosphoric Acids
  • Resin Cements
  • Silicon Compounds
  • casein phosphopeptide-amorphous calcium phosphate nanocomplex
  • clearfil majesty
  • Water
  • carbopol 940
  • Diamond
  • Edetic Acid
  • phosphoric acid
  • silicon carbide