A simplified quantitative test--adapted Checkbuf test--for resting saliva buffering capacity compared with a standard test

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Oct;108(4):551-6. doi: 10.1016/j.tripleo.2009.06.008. Epub 2009 Aug 28.

Abstract

Objective: The aim of this study was to adapt a quantitative test for stimulated saliva, known as the Checkbuf test, to measure the buffering capacity of resting saliva and to evaluate its correlation with the Ericsson test for resting saliva.

Study design: Resting saliva was collected from 112 patients. Buffering capacity was assessed with the adapted Checkbuf test and 4 other methods: Ericsson test for resting saliva, Checkbuf test, and commercially available paper and liquid colorimetric tests for stimulated saliva. Data were analyzed by Cohen kappa coefficient to evaluate the agreement between the Ericsson test and the other tests.

Results: There was a significant positive agreement between the Ericsson test for resting saliva and adapted Checkbuf test (kappa = 0.708). However, there were no agreements between the Ericsson test and the 3 other tests.

Conclusions: This study demonstrated that the adapted Checkbuf test is useful for measuring resting saliva buffering capacity, even for subjects with low salivary flow rates.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Buffers
  • Colorimetry / instrumentation
  • Colorimetry / methods
  • Drug Therapy
  • Female
  • Humans
  • Hydrogen-Ion Concentration
  • Male
  • Middle Aged
  • Reagent Kits, Diagnostic / classification
  • Reagent Kits, Diagnostic / standards
  • Reference Standards
  • Saliva / physiology*
  • Secretory Rate / physiology
  • Xerostomia / physiopathology
  • Young Adult

Substances

  • Buffers
  • Reagent Kits, Diagnostic