Abstract
A monoclonal antibody, McAb9E (IgG3), was generated against a metastatic HCC cell line, MHCC-1. The antigen was characterized as human Caveolin-1 (Cav-1, 21kDa), with pI of 5.65. The Cav-1 antigen was found significantly over expressed in metastatic HCC cell lines as well as in tumor specimens. The Cav-1 specific McAb may be a useful molecular agent for metastatic HCC.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / analysis*
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / immunology*
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Antibody Specificity
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Carcinoma, Hepatocellular / immunology
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Carcinoma, Hepatocellular / pathology*
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Caveolin 1 / immunology*
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Cell Line, Tumor
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Gene Expression Regulation, Neoplastic
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Humans
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Molecular Sequence Data
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Neoplasm Metastasis
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Proteomics*
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Tandem Mass Spectrometry
Substances
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Antibodies, Monoclonal
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Caveolin 1