BODIPY-based fluorescent probe for peroxynitrite detection and imaging in living cells

Zhen-Ning Sun; Hua-Li Wang; Feng-Qin Liu; Yan Chen; Paul Kwong Hang Tam; Dan Yang

doi:10.1021/ol900279z

BODIPY-based fluorescent probe for peroxynitrite detection and imaging in living cells

Org Lett. 2009 May 7;11(9):1887-90. doi: 10.1021/ol900279z.

Authors

Zhen-Ning Sun¹, Hua-Li Wang, Feng-Qin Liu, Yan Chen, Paul Kwong Hang Tam, Dan Yang

Affiliation

¹ Morningside Laboratory for Chemical Biology, Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.

PMID: 19331349
DOI: 10.1021/ol900279z

Abstract

A fluorescent probe, HKGreen-2, has been developed based on a specific reaction between ketone and peroxynitrite (ONOO(-)). This probe is highly sensitive and selective for the detection of peroxynitrite not only in abiotic but also in biological systems. With this probe, we successfully detected peroxynitrite generated in murine macrophage cells activated by phorbol 12-myristate 13-acetate (PMA), interferon-gamma (IFN-gamma), and lipopolysaccharide (LPS). This new probe will be a useful tool for studying the roles of peroxynitrite in biological processes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Boron Compounds / chemical synthesis*
Boron Compounds / chemistry*
Fluorescent Dyes / chemical synthesis*
Fluorescent Dyes / chemistry
Interferon-gamma / pharmacology
Lipopolysaccharides / pharmacology
Mice
Models, Molecular*
Molecular Structure
Peroxynitrous Acid / analysis*
Spectrometry, Fluorescence
Tetradecanoylphorbol Acetate / pharmacology

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Fluorescent Dyes
HKGreen-2
Lipopolysaccharides
Peroxynitrous Acid
Interferon-gamma
Tetradecanoylphorbol Acetate