Roles of neutrophil gelatinase-associated lipocalin in continuous ambulatory peritoneal dialysis-related peritonitis

Joseph C K Leung; Man Fai Lam; Sydney C W Tang; Loretta Y Y Chan; K Y Tam; Terence P S Yip; Kar Neng Lai

doi:10.1007/s10875-008-9271-7

Roles of neutrophil gelatinase-associated lipocalin in continuous ambulatory peritoneal dialysis-related peritonitis

J Clin Immunol. 2009 May;29(3):365-78. doi: 10.1007/s10875-008-9271-7. Epub 2009 Jan 16.

Authors

Joseph C K Leung¹, Man Fai Lam, Sydney C W Tang, Loretta Y Y Chan, K Y Tam, Terence P S Yip, Kar Neng Lai

Affiliation

¹ Department of Medicine, Queen Mary Hospital, University of Hong Kong, Room 409, Professorial Block, 102 Pokfulam Road, Hong Kong, China.

PMID: 19148711
DOI: 10.1007/s10875-008-9271-7

Abstract

Introduction: We measured the neutrophil gelatinase-associated lipocalin (NGAL) concentration in peritoneal dialysate effluent (PDE) collected following an acute episode of continuous ambulatory peritoneal dialysis (CAPD)-related peritonitis.

Results: NGAL concentration in PDE increased in the first 3 days after developing peritonitis and correlated well with the neutrophil count. In patients with culture-negative peritonitis, the NGAL in PDE was lower than that in patients with gram-positive or gram-negative peritonitis. Apart from providing additional diagnostic support to bacterial-induced peritonitis, measurement of NGAL in PDE may be useful to differentiate the neutrophil-dependent culture-negative peritonitis from that associated with non-bacterial or non-cellular etiologies.

Conclusion: Human peritoneal mesothelial cell (HPMC) is another source of NGAL during peritonitis. NGAL was specifically induced in HPMC by IL-1beta. Incubation of HPMC with recombinant NGAL reversed the transforming growth factor-beta-induced up-regulation of Snail and vimentin but rescued the down-regulation of E-cadherin. Our data suggest that NGAL may exert a protective effect in modulating the epithelial-to-mesenchymal transition activated following peritonitis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute-Phase Proteins / genetics
Acute-Phase Proteins / immunology
Acute-Phase Proteins / metabolism*
Adult
Ambulatory Care
Biomarkers / metabolism
Cadherins / metabolism
Cell Movement
Cells, Cultured
Early Diagnosis
Gene Expression Regulation
Humans
Interleukin-1beta / metabolism
Kidney Failure, Chronic / complications
Kidney Failure, Chronic / therapy
Lipocalin-2
Lipocalins / genetics
Lipocalins / immunology
Lipocalins / metabolism*
Male
Middle Aged
Neutrophils / immunology
Neutrophils / metabolism*
Neutrophils / pathology
Peritoneal Dialysis / adverse effects*
Peritonitis / diagnosis
Peritonitis / etiology*
Peritonitis / metabolism*
Peritonitis / pathology
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / immunology
Proto-Oncogene Proteins / metabolism*
Snail Family Transcription Factors
Streptococcal Infections / etiology*
Streptococcal Infections / immunology
Streptococcal Infections / metabolism*
Streptococcus*
Transcription Factors / metabolism
Transforming Growth Factor beta / metabolism
Vimentin / metabolism

Substances

Acute-Phase Proteins
Biomarkers
Cadherins
Interleukin-1beta
LCN2 protein, human
Lipocalin-2
Lipocalins
Proto-Oncogene Proteins
Snail Family Transcription Factors
Transcription Factors
Transforming Growth Factor beta
Vimentin