Abstract
The role of the N-terminal region of the transcription factor FNR, which immediately precedes the first ligand (Cys20) of the [4Fe-4S] cluster, was investigated. We found that truncation mutants that removed residues 2 to 16 and 2 to 17 had wild-type levels of FNR protein but surprisingly altered O(2) regulation.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Aerobiosis
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Amino Acid Substitution
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Iron-Sulfur Proteins / genetics
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Iron-Sulfur Proteins / metabolism*
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Mutagenesis, Site-Directed
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Mutation
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Oxygen / metabolism
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Promoter Regions, Genetic
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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DNA-Binding Proteins
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Escherichia coli Proteins
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FNR protein, E coli
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Iron-Sulfur Proteins
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Transcription Factors
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Oxygen